Genome-wide association mapping reveals that specific and pleiotropic regulatory mechanisms fine-tune central metabolism and growth in arabidopsis
Fusari, Corina M. Kooke, Rik Lauxmann, Martin A. Annunziata, Maria Grazia Encke, Beatrice Hoehne, Melanie Krohn, Nicole Becker, Frank F.M. Schlereth, Armin Sulpice, Ronan
Fusari, Corina M.
Kooke, Rik
Lauxmann, Martin A.
Annunziata, Maria Grazia
Encke, Beatrice
Hoehne, Melanie
Krohn, Nicole
Becker, Frank F.M.
Schlereth, Armin
Sulpice, Ronan
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Date
2017-09-27
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journal article
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Citation
Fusari, Corina M. Kooke, Rik; Lauxmann, Martin A.; Annunziata, Maria Grazia; Encke, Beatrice; Hoehne, Melanie; Krohn, Nicole; Becker, Frank F.M.; Schlereth, Armin; Sulpice, Ronan; Stitt, Mark; Keurentjes, Joost J.B. (2017). Genome-wide association mapping reveals that specific and pleiotropic regulatory mechanisms fine-tune central metabolism and growth in arabidopsis. The Plant Cell 29 (10), 2349-2373
Abstract
Central metabolism is a coordinated network that is regulated at multiple levels by resource availability and by environmental and developmental cues. Its genetic architecture has been investigated by mapping metabolite quantitative trait loci (QTL). A more direct approach is to identify enzyme activity QTL, which distinguishes between cis-QTL in structural genes encoding enzymes and regulatory trans-QTL. Using genome-wide association studies, we mapped QTL for 24 enzyme activities, nine metabolites, three structural components, and biomass in Arabidopsis thaiiana. We detected strong cis-QTL for five enzyme activities. A cis-QTL for UDP-glucose pyrophosphorylase activity in the UGP1 promoter is maintained through balancing selection. Variation in acid invertase activity reflects multiple evolutionary events in the promoter and coding region of VAC-INV. cis-QTL were also detected for ADP-glucose pyrophosphorylase, fumarase, and phosphoglucose isomerase activity. We detected many trans-QTL, including transcription factors, E3 ligases, protein targeting components, and protein kinases, and validated some by knockout analysis. trans-QTL are more frequent but tend to have smaller individual effects than cis-QTL. We detected many colocalized QTL, including a multitrait QTL on chromosome 4 that affects six enzyme activities, three metabolites, protein, and biomass. These traits are coordinately modified by different ACCELERATED CELL DEATH6 alleles, revealing a trade-off between metabolism and defense against biotic stress.
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American Society of Plant Biologists (ASPB)